Isolation and Characterization of Brucella spp., Low-Density Polyethylene (LDPE) Plastic Degrading Bacteria in Al-Ahsa Region, Saudi Arabia

Plastic pollution is one of the most serious environmental issues, causing severe damage. It vital importance to find an efficient and eco-friendly approach biodegrading plastics. The aim this study was isolate characterize different bacterial isolates from water samples in Al-Ahsa region Saudi Arabia. ability degrade low-density polyethylene (LDPE) plastic evaluated using multiple approaches, including changes media pH values, weight loss, Fourier transform infrared (FTIR), gas chromatography–mass spectrometry (GC–MS). were collected plastic-contaminated sites Al-Ahsa, obtained a mineral nutrient medium (MNM) enriched with LDPE as only carbon energy source. Two (APCK5 APCZ14) they showed potential degradation, evidenced by (from 7.0 ± 0.03 6.17 0.05 6.22 0.03), reduction (8.1 0.63% 18.85 0.96%, respectively), FTIR GC–MS analyses. Based on 16S rRNA gene similarities, APCZ14 APCK5 determined be closely related genus Brucella. exhibited 99.48% homology Brucella cytisi, whereas 99.33% similarity level tritici. In conclusion, both strains had high efficiency biodegradation could developed for wide use method remove or reduce pollutants environment.